Studies on Properties of Immobilized Cellulase on a Carrier

DONG Xue-wei; ZttU Qi-zhong; YU Xiu-min; LV Xin-ping; XU Guo-ying; YU Tao

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Studies on Properties of Immobilized Cellulase on a Carrier

更新时间：2026-07-10

- Studies on Properties of Immobilized Cellulase on a Carrier
- Vol. 13, Issue 5, Pages: 118-122(2008)
- 作者机构：
  
  [1]山东大学威海分校海洋学院,山东,威海,264209
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2008
- 稿件说明：
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DONG Xue-wei, ZttU Qi-zhong, YU Xiu-min, et al. Studies on Properties of Immobilized Cellulase on a Carrier[J]. 2008, 13(5): 118-122.
DOI：

DONG Xue-wei, ZttU Qi-zhong, YU Xiu-min, et al. Studies on Properties of Immobilized Cellulase on a Carrier[J]. 2008, 13(5): 118-122. DOI：

摘要

摘　　要：采用交联法制备了聚乙二醇改性壳聚糖（PEG—CS）载体，并将其用于固定纤维素酶．探讨了载体制备过程中的几种主要影响因素、纤维素酶固定化的影响因素，并对固定化纤维素酶的性质进行了讨论．结果表明，固定化酶最适pH值比原酶降低，最适温度为60℃，比游离酶高10℃．在pH值为5．0，温度40℃时，固定化酶对羧甲基纤维素钠盐的表观米氏常数为7．2mg／L，而游离酶为3．3mg/L．与游离酶相比，该固定化纤维素酶热稳定性明显提高，并具有良好的操作和存储稳定性．

Abstract

PEG- CS（ chitosan modified by polyethylene glycol） was prepared and applied to immobilize cellu- lase. ＇lhe immobilization conditions and properties of the immobilized enzyme on PEG - CS were studied. The immobilized enzyme exhibits the maximal activity at pH 4. 0. The optimal temperature of immobilized enzyme is at 60~

higher than free eellulase. In acetic acid-sodium-acetate buffer（ pH 5.0

40℃ ）

the Km for CMCNa of immobilized cellulase and free cellulase are separately 7.2mg/L and 3.3mg/L

so the affinity of cellulase decreases after being immobilized. The thermal-

operational-

and storage-stabilities of the immobilized enzyme are obviously increased compared to those of the free one.

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