ZHAO Xinyue, MA Fang, YANG Jixian, et al. Isolation of an atrazine-degrading bacterium (Pseudomonas sp. ZXY-1) and its biodegradation and kinetic studies[J]. 2018, 50(2): 2018-2.
DOI:
ZHAO Xinyue, MA Fang, YANG Jixian, et al. Isolation of an atrazine-degrading bacterium (Pseudomonas sp. ZXY-1) and its biodegradation and kinetic studies[J]. 2018, 50(2): 2018-2.DOI: 10.11918/j.issn.0367-6234.201612088.
Isolation of an atrazine-degrading bacterium (Pseudomonas sp. ZXY-1) and its biodegradation and kinetic studies
A new atrazine-degrading strain named ZXY-1 was isolated in the present study. ZXY-1 was identified as Pseudomonas based on the physio-biochemical characteristics and 16s rRNA gene sequence analysis. The newly isolated strain had a strong ability to biodegrade atrazine of 100 mg/L completely within 11 h
with a degrading rate up to 9.09 mg/(L·h). The optimal conditions for atrazine degradation were captured: temperature 30 ℃
pH 8.0
velocity of shaker 150 r/min and inoculums 3%. Atrazine biodegradation
under the optimum conditions
fitted the first-order kinetic equation when the initial atrazine concentration ranged from 20 mg/L to 80 mg/L
and the zero-order kinetic equation when the initial atrazine concentration ranged from 100 mg/L to 250 mg/L. The kinetic analysis indicated that the strain ZXY-1 cell growth perfectly suited Haldane model with μmax
Ks and Ki of 0.696 h-1
98.55 and 142.6 mg/L
respectively. Overall
this study provided an efficient bacterium and approach that might serve as a viable bioremediation solution to treat the atrazine wastewater.