Abstract:Microbial Fuel Cell (MFC) was constructedusing phenolcontaining anaerobic wastewater as a substrate. An anaerobicelectrogenic strain A4 capable of degrading phenol was obtained by separatingand screening the stable period microbial fuel cell anode. Physiological andbiochemical identification of strain A4 showed that the strain wasEnterobacter. Used strain A4 as the research object

the effects of differentnitrogen sources

carbon sources

pH value

temperature

initial phenolconcentration and heavy metal ion on the growth of strain A4 and phenoldegradation were investigated. Studies have shown that NH4NO3 was the best nitrogen source

sucrose was the best cometabolic substrate

the optimum pH value was 8

theoptimal initial phenol concentration was 500 mg/L

the optimum temperature was23℃

and the optimum inoculum was 10%. Strain A4 could tolerate acertain concentration (0.2 mg/L) of Pb2+

while this strain was lesstolerant to high concentrations (2000 mg/L) of phenol. Strain A4 producedelectricity during the stationary phase of growth. The phenol degradation ratereached 67.37% at 64h and the coulombic efficiency (CE) was 17.8%. The research selected highqualityanaerobic electricityproducing bacteria and optimized their growth conditions

which is of great significance for the application of MFC in the treatment ofphenolcontaining wastewater.