Study on Ligninase Activity of Aspergillus fumigatus G13 Induced by Lignin Model Compounds

FENG Qian; YAN Hong

doi:10.15938/j.jhust.2020.02.021

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Study on Ligninase Activity of Aspergillus fumigatus G13 Induced by Lignin Model Compounds

更新时间：2026-08-31

- Study on Ligninase Activity of Aspergillus fumigatus G13 Induced by Lignin Model Compounds
- Vol. 25, Issue 2, Pages: 156-165(2020)
- 作者机构：
  
  1. 1哈尔滨理工大学化学与环境工程学院,哈尔滨,150040
  2. 2黑龙江省高校绿色化工技术重点实验室,哈尔滨,150040
- 作者简介：
- 基金信息：
- DOI：10.15938/j.jhust.2020.02.021
  CLC： O6433;Q9399
- Published：2020
- 稿件说明：
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FENG Qian, YAN Hong. Study on Ligninase Activity of Aspergillus fumigatus G13 Induced by Lignin Model Compounds[J]. 2020, 25(2): 156-165.
DOI：

FENG Qian, YAN Hong. Study on Ligninase Activity of Aspergillus fumigatus G13 Induced by Lignin Model Compounds[J]. 2020, 25(2): 156-165. DOI： 10.15938/j.jhust.2020.02.021.

摘要

摘要:以木质素酶活力作为指标，采用阿魏酸、对香豆酸和芥子酸3种模型化合物作为底物，通过添加纤维素及葡萄糖并改变培养方式，研究AFumigatusG13诱导产酶特性。结果表明，摇床培养较静置培养菌株产酶能力提高了2~5倍；单独采用木质素模型化合物作为诱导物，芥子酸诱导作用最强，其LiP、Lac和MnP最大酶活分别可达24.08、21.67、110.78.U/L；羧甲基纤维素、微晶纤维素和葡萄糖作为共底物，均对3种模型化合物对菌株的诱导能力起促进作用，其中葡萄糖的促进作用最为明显（较原酶活提高约1.38~5.34倍），羧甲基纤维素的促进效果优于微晶纤维素；且纤维素模型化合物和葡萄糖在诱导产酶方面起协同作用。

Abstract

Abstract:With the ligninase activity as anindex

three model compounds

ferulic acid

pcoumaric acid and sinapic acid

were used as substrates to study the characteristics of Aspergillus fumigatus G13induced enzyme production by adding cellulose and glucose and changing theculture mode The results showed that the ability of producing enzymes in shakeflask culture was 2-5 times higher than that in static culture The lignin modelcompound was used as inducer alone

and the induction effect of sinapic acidwas the strongest The maximum activities of LiP

Lac and MnP were 2408

2167and 11078U/L

respectively Carboxymethyl cellulose (CMC)

microcrystallinecellulose (MCC) and glucose as cosubstrates all promoted the induction abilityof the three model compounds to the strain Among them

glucose was the mostprominent (about 138-534 times higher than the original enzyme activity)

andCMC was better than microcrystalline cellulose Cellulose model compounds andglucose played a synergistic role in the induction of enzyme production

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Related Author

FENG Qian

GUO Wei

YAN Hong

Related Institution

2Key Laboratory of Green Chemical Technology of College of Heilongjiang Province

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