In biological and medical research

two-vessel occlusion (2-VO) is often used to establish cerebral ischemia models

while due to the non-quantitative ligation of the artery

the model usually has poor reproducibility

large individual differences within the group

and high mortality. In this paper

a vascular blocking tension meter is proposed by taking metal strain gauge as the core component to realize the quantitative detection of ligation force. Biological experimental verification was carried out in vitroand in vivo

as well as in such aspects as cells and molecules level

tissues and organs level

and overall level. Results show that the linearity and sensitivity of the tension meter were affected by factors such as base parameters

bonding process

circuit parameters

and appearance structure of the metal strain gauge. Compared with the non-instrumental ligation group

the animal mortality of the cerebral ischemia model was reduced by 42.22%. The decrease in cerebral blood flow of the experimental animals corresponded to the increase in ligation force. After hematoxylin-eosin (HE) staining of the hippocampus

light microscopy results show that the neurons were loosely arranged

the number of neurons decreased and even disappeared

the cytoplasmic staining of the neurons became weak

and the dentate gyrus exhibited neuronal pyknosis. A comparison between different ligation force groups shows that the individual differences in the hippocampus morphology of the rats in the 1.5 N ligation force group were the smallest. Morris water maze results show that compared with the sham operation group

the escape latencies of the rats in the 1.5 N ligation group and the 2.5 N ligation group were significantly increased (P<0.05)

and the number of platform crossing times in the 1.5 N ligation group was significantly decreased (P<0.05). There was an obvious learning and memory disorder. It was falsified that the greater the ligation force of the 2-VO rats was

the better the ligation effect was

which was obtained in our previous laboratory experiments. The conclusion was drawn that the smaller the standard deviation of the ligation force was

the better the ligation effect was

and the ligation force could be quantitatively controlled. The application of the vascular blocking tension meter to the basic experimental research of medicine is of significance.