Reed cellulose pulp was pretreated by ultrasonic，microwave，N，N一dimethylara acetamide(DMAc ) and tetrahydrofuran(THF)，respectively. Reed microcrystalline cellulose(MCC)was prepared by thedilute hydrochloric acid hydrolysis from the reed which pretreated by different methods. The structure and propertiesof MCC were characterized by XRD，FT-IR and TGA. The results showed that the crystallinity of MCC with fourpretreatment methods was 68. 45 %，62. 28 %，63.21 and 69. 56%，respectively. The thermal stability of MCCprepared by hydrolysis after pretreated by ultrasonic was the best compared with other three methods. The yield ofMCC with ultrasonic pretreatment was the highest. The foundation for the further research and application of MCCwas laid.